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The TNF- levels were determined using the rat TNF- ELISA kit from Boster Biological Technology (CA, United States), while IL-1 and IL-6 levels were measured using the rat IL-1 beta/IL-1F2 Quantikine ELISA Kit and the rat IL-6 Quantikine ELISA Kit from R&D Systems (MN, United States), respectively

doi: 10.1016/s0738-081x(02)00225-0

The containers are treated and disposed of

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