Four m-thick longitudinally-oriented sections including metaphysis were deparaffinized and rehydrated, and then processed for hematoxylin and eosin (H&E) (Thermo Scientific), tartrate resistant acid phosphatase (TRAP) staining (Sigma-Aldrich) and immunohistochemical staining using primary antibodies against Osterix (Abcam, 1:200 dilution) or Osteocalcin (Takara, 1:200 dilution)
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World journal of gastroenterology , 23 (48), 8465-8488