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Gibco, Life technologies) at 37 C in the presence of 5% CO 2 , without or with selective concentrations of colchicine or doxorubicin (5 g/ml for CHO R C5 and 400 nM for MDA-Doxo 400 cells

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For INS-1 832/3, agonists were added in HBSS (acute incubations) or complete medium (prolonged incubations), at 11 mM glucose, at the time of seeding into plates

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Minimizing the risk of developing serious complications