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Cells were cultured in Dulbeccos Modified Eagle Medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 100 g/mL streptomycin and maintained at 37 C in a humidified atmosphere containing 5% CO

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An In Situ Cell Death Detection Kit (Merck Life Sciences, Madrid, Spain) was subsequently used to detect DNA strand breaks following the manufacturers instructions