For the reaction, 2 g of the total RNA were combined with water pretreated with diethylpyrocarbonate (DEPC-H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and with 1 L of Oligo(dT) 15 primer (0.5 g/reaction, Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 5 min at 70 C
10.7150/thno.49069 98 KarS.GajewiczA.PuzynT.RoyK.LeszczynskiJ
PFOA has a long half-life that may last for several years (1.2 to 14.9 years) (1721)
Most experiments studying osteoclast differentiation induce osteoclastogenesis in human or rodent myeloid cells via treatment with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappa- ligand (RANKL), a process that is inhibited with dopamine treatment
10.1249/MSS.0000000000002800 149 KangJ