The sections were blocked for 1 h at room temperature with 5% normal goat serum and stained with anti-atrial natriuretic peptide (ANP) antibody (Servicebio, Wuhan, China) diluted 1: 200, anti-myosin heavy chain 7(MYH7) antibody (Proteintech Group, Inc, Wuhan, China) diluted 1: 200, anti-cytochrome c oxidase subunit II (COX II) antibody (Cell Signaling Technology, Inc, Boston, USA) diluted 1: 1000, anti-NLRP3 antibody diluted (ABclonal, Wuhan, China) 1: 200, and anti- interleukin-18 (IL-18) antibody (Servicebio, Wuhan, China) diluted 1: 1000 overnight at 4 C
If you miss a dose, you should administer the injection as soon as you remember, provided its within five days of the missed date
Always wash your hands and keep the injection site clean Wash your hands before you start, and use soap and water or an alcohol swab to clean the injection site
Supplements that complement tirzepatide can address nutritional gaps that emerge from reduced food intake
Document consent, counselling and outcomes in the patient record
