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Quantitative Real-time-PCR (qRT-PCR) analysis The relative ovarian SIRT1, steroidogenic acute regulatory protein (STAR), cytochrome P450 17A1 (CYP17A1), hydroxysteroid (17) dehydrogenase type 3 (HSD17B3), nuclear factor erythroid 2 related factor 2 (Nrf2), mitochondrial DNA (mtDNA), forkhead box protein O1 (FOXO1), nuclear factor kappa B (NF-B), and inducible nitric oxide synthase (iNOS) mRNA abundance was determined by qRT-PCR using SYBR Green PCR Master Mix (2x SensiFast SYBR, Bioline, catalog No

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Intensive insulin therapy Although insulin resistance is a major contributor to the development of NAFLD in most cases [40], insulin therapy has not been proved to resolve or improve NAFLD