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The hormone circuit mentioned is composed of the ovaries , the adrenal gland, and the thyroid gland

DSIP is a nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) first isolated from rabbit cerebral venous blood during slow-wave sleep induction by Monnier et al

Goldfish kisspeptin: Molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolactin, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level

Firefly and Renilla luciferase activities were measured according to the manufacturers guidelines (Beyotime, RG088S)