Ther Adv Endocrinol
Cooling to 0 , stirring for 30min, adding 0.22g of HOSu, stirring and reacting for 12 hours at the temperature, filtering, collecting filtrate, and spin-drying the filtrate to obtain a crude Boc-His (Trt) -Aib-OSu product
Relative protein expression was analyzed using -actin (dilution 1:5000
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