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Electrophysiological recordings Coronal 250- to 300-m slices containing the LS were prepared using a vibratome (Leica, VT-1000S) in an ice-cold choline-based solution containing (in mM) 110 choline chloride, 2.5 KCl, 0.5 CaCl 2 , 7 MgCl 2 , 1.3 NaH 2 PO 4 , 1.3 Na-ascorbate, 0.6 Na-pyruvate, 25 glucose, and 25 NaHCO 3 , saturated with 95% O 2 and 5% CO 2

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