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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)

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Thematic review series: glycerolipids

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However, on the other hand, studies on the interaction of different cells in the microenvironment have found that CAFs cells have a high metabolism of BCAA and provide BCKA to PDAC cells to assist tumor cells in BCAA metabolism