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GSH depletion lowered IC 50 values for both AQ-QI (from 54 to 20 M) and DEAQ-QI (from 40 to 18 M)

Sinn N, Milte C, Howe PRC

After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

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In contrast, pathogen-associated molecular patterns (PAMPs) are conserved molecular structures found in certain microbes for the innate immunity, leading to responses such as cytokine and interferon production, helping to protect the host from infection