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KGN were maintained in Dulbeccos modified Eagles medium (DMEM, HyClone, USA, #SH30022.01) medium (Invitrogen) containing 10% ( v / v ) fetal bovine serum and 1% ( v / v ) penicillin/streptomycin

Butyrate serves as a primary energy source for colonocytes and enhances intestinal barrier integrity by increasing the expression of tight junction proteins like occludin, which is vital for preventing systemic inflammation 34