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The BUTE-CM supernatant was extracted and stored in 80 C until future ultracentrifugation or subject to immediate ultracentrifugation using a Beckman Coulter L-80 Optima Ultracentrifuge at 4 C

Daily routine: Morning: Topical GHK-Cu to scalp Evening: Topical GHK-Cu to scalp Every day: BPC-157 500mcg subcutaneous injection 3x per week: TB-500 2-5mg subcutaneous injection (maintenance dose) Bedtime: IGF-1 LR3 50-60mcg (4-6 week cycles with breaks) Additional: Weekly microneedling with GHK-Cu application immediately after Monthly progress photos and measurements Expected results: This is the most aggressive protocol

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However, even with this safer technique, there are reports of dyschromia after procedures with short intervals [123]

After 15 min, mitochondria were incubated with 1 mM NADH