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The cell suspension was spun down at 14,000 g for 10 minutes, and 3 l of supernatant was applied to an Accucore 150 Amide HILIC HPLC column (100 2.1 mm, 2.6-m particle size) equipped with a guard cartridge (at 30 C)

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Features that should prompt this diagnosis include Spitzoid cellular morphology with age greater than 10 years, diameter greater than 10 mm, ulceration, involvement of the subcutaneous fat, and greater than six mitoses per mm 2 [108]

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