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Introduce gradually over 710 days if needed

4, remove the sealing film, discard the liquid, pat dry on absorbent paper, fill each well with washing solution, and let it stand1min, shake off the detergent, pat dry on absorbent paper, and repeat this process.5If you use an automatic plate washer, please follow the plate washer operating procedures to wash the plate and add soaking30sAfter washing the plate, pat the plate dry on a clean, lint-free paper before adding the substrate

Lipparini, F

Consent for Publication The parents of the patient provided written informed consent for the collection of data and the publication of this case report, including accompanying images

Nature Methods 3 , 715719 (2006)