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Image reconstructions were conducted using Hiscan Reconstruct software (Hiscan Medical Technology Co., Ltd., China), while quantitative morphological analysis was accomplished using Hiscan Analyzer software (Hiscan Medical Technology Co., Ltd., China)

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In unstimulated HUVECs, VWF was observed in the endothelial cytoplasm but not on the cells surfaces, whereas in stimulated vessels, long VWF fibers up to 2 mm in length covered the vessel surfaces, binding to platelets predominantly irreversibly