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Substance Use Disorder: Inhaling drugs through the nose over long periods of time can damage the internal nasal structures, leading to the breakdown of the cartilage Cadogan Clinic

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Borgonetti V, Vozella V, Ware T, Cruz B, Bullard R, Cravatt BF, Galeotti N, Roberto M (2024) Excessive alcohol intake produces persistent mechanical allodynia and dysregulates the endocannabinoid system in the lumbar dorsal root ganglia of genetically-selected Marchigian Sardinian alcohol-preferring rats

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The cells were cultured overnight as in step 2