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Nutritional Interventions: Hypothyroid patients may benefit from specific dietary approaches: Caloric deficit : Modest reduction of 500-750 calories daily, targeting 1-2 pounds weekly weight loss Protein optimization : 1.2-1.6 g/kg body weight to preserve lean mass during weight loss (adjust for patients with chronic kidney disease under medical supervision) Anti-inflammatory diet : Mediterranean-style eating patterns may benefit patients with autoimmune thyroiditis Micronutrient attention : Adequate iodine intake (RDA 150 mcg/day

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Tirzepatide, a dual GLP-1/GIP receptor agonist that has been approved for controlling blood sugar in T2DM, has emerging evidence suggesting that it is superior to GLP-1RAs in terms of glycemic control and weight loss (30, 31)

For example, it increases collagen and elastin synthesis, stimulates nerve and blood vessel outgrowth, and can enlarge human hair follicle size

Restriction enzymes and T4 DNA ligase were obtained from New England Biolabs