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Avoid normal saline (0.9% sodium chloride solution)

Following 16 h incubation (at 4 C), the wells were washed with PBS/0.1% BSA and the bound radioactivity (representing 3 H-25HC bound to v3 integrin in the absence and presence of fractalkine-CD protein) was counted using a TRI-CARB 2900TR liquid scintillation counter

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