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W., Bridges, R

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Unlike muscles or skin, discs have very limited blood supply

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The retained protein fraction was immediately used for the determination of -glutamylcysteine concentration or assayed for GCL activity in 0.1 M TrisHCl buffer containing 0.15 mM KCl, 20 mM MgCl 2 , 2 mM EDTA, 10 mM ATP, 10 mM L-cysteine, 40 mM L-glutamate and 220 M acivicin, pH 8.2, at 37 C for 15 min