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International Journal of Current Microbiology and Applied Sciences, 7, 48184823

They may also worry about potential effects on mental health, the liver or risk of inflammatory bowel disease (IBD)

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STAMPING: Sanitize the Needle cap by spraying or dipping it in rubbing alcohol for 30 seconds before each use

Cells were lysed for 15 minutes in ice-cold RIPA buffer consisting of 1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP (Sigma Cat#4906845001)