High resistance to cisplatin in human ovarian cancer cell lines is associated with marked increase of glutathione synthesis
Moreover, in breast cancer, IGF-1 mainly upregulates cysteine-rich 61 (Cyr61) by activating the PI3K/AKT pathway and an increase in Cyr61 promotes the growth and invasion of breast cancer cells (27)
In the technical scheme, in the step 3, the prepared solution is dissolved and clarified by stirring, the injection water is added to the full amount, and the pH value is adjusted to 6.9 by 10% sodium hydroxide solution
For microdosing protocols , some researchers add 2.5 mL to a 5 mg vial, creating a 2 mg/mL solution
After 3 days in the presence of unlabelled GSH ( t = 72 h), cells were extracted for metabolomic analysis as described above