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Featured applications: Maintenance of pluripotency in iPSC Cell culture media optimization and replacement of insulin in defined media IGF-1 LR3 activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells

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To further support the impact of CBS on replenishing GSH levels under cystine-restricted conditions, we expressed a codon-optimized CBS (CBS OPT ), which is resistant to the designed sgRNA (Fig

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