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How many sets should you push to failure, and should you do it every time

In: Erdman JW, Macdonald IA, Zeisel SH, editors

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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