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Five micrograms of total RNA was subjected to reverse transcription using the Superscript III first-strand synthesis system (Invitrogen) according to the manufacturer's instructions

Aging Cell 21 , e13646 (2022)

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Active Constituents: Zinc 40 mg/ml (as disodium zinc EDTA), manganese 10 mg/ml (as disodium manganese EDTA), copper 15 mg/ml (as copper disodium EDTA), selenium 5 mg/ml (as sodium selenite) and 1.4 mg/ml Vitamin B12 (cyanocobalamin)