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Balaj says that when he started working with the peptide back in 2017, the information about protocols was not so clear

Free Radical Res (2020) 54:126

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Indeed, mutant OPG proteins expressed in co-cultured OPG-deficient osteoblasts retain their ability to bind RANKL but lack the ability to regulate RANKL transport and could not restore osteoclast formation to wild-type levels, suggesting that osteoclastogenic capacity is at least partially regulated by OPG/RANKL (Honma et al., 2013