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These functional declines are compounded by dysfunctional signaling in TLR2 and TLR4 pathways, further impairing immune responses in the elderly [259]

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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

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