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The crude residue was purified by silica gel flash column chromatography ( n -heptane:EtOAc, 2:1) yielding 4-nitrophenyl (2-(pyridin-2-yldisulfaneyl)ethyl) carbonate (89%)

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The difference in amino acid composition may be related to the physiological needs of ice age animals to adapt to cold environments.The increase of hydrophobic amino acids may help enhance the stability and frost resistance of proteins, allowing GH receptors to maintain their normal structure and function in low-temperature environments

Yes, your Majesty