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used the transcriptome and metabolome analysis to investigate the effect of DDG on the spermatogenesis in testis and ovulation in ovary in zebrafish, respectively, which is important to assess the ecological risks of DDG in the environment in fish

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The lid was opened to volatilize off the excess ethanol, which is then added to diethyl pyrocarbonate water (NR0001, Regen, China) to be stored in 80C

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