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Data extraction A predetermined and structured data extraction form was used

The results suggested that the mitochondria in the blank group were morphologically more intact, with a rod-like morphology, surrounded by a double-layered membrane, with the inner membrane protruding inwards to form flat plate-like cristae, and that the cristae of the mitochondria were continuous

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2.3 16S rRNA gene sequencing, read processing and annotation The V3-V4 region of the 16S rRNA gene was amplified with forward primer 5-TCGT CGGC AGCG TCAG ATGT GTAT AAGA GACA GCCT ACGG GNGG CWGC AG-3 and reverse primer 5-GTCT CGTG GGCT CGGA GATG TGTA TAAG AGAC AGGA CTAC HVGG GTAT CTAA TCC-3, with adapter sequences for compatibility with the Illumina Nextera XT Index kit

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