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Gene expression and Western blotting studies confirmed the selective deletion of Barr1 in pancreatic islets of TMX-injected fl/fl Barr1 Pdx1-Cre-ER TM mice (Supplemental Figure 1A, C)

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Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h