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pH 7) 1x Regeneration Buffer (0.1 M Glycine/HCl, pH 2.8) 1x Blocking/Elution Buffer (2 mM D-biotin in PBS) 2

Additionally, the expression of mRNA and protein for both 25-hydroxylases (CYP2R1 and CYP27A1), 1--hydroxylase (CYP27B1), and VDR were downregulated, but that of 24-hydroxylase (CYP24A1) was significantly upregulated in the liver of HFD-fed mice compared to those in controls (Fig

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Aierken et al

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