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The skinned LV sections were then washed and homogenized in a relaxing solution with protease inhibitors (10 mM Na 2 EGTA

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In particular, unlike the conventional method, the method of the present invention can achieve preparation of (R)-4-chloro-3-hydroxy butyric acid alkyl ester having an optical purity of more than 99%ee from the hydrolysis of the racemic 4-chloro-3-hydroxy butyric acid alkyl ester using the enzyme