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The best time to inject is 30 to 45 minutes before bedtime, on an empty stomach, ideally 2 to 3 hours after your last meal

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butyricum improved intestinal barrier integrity by increasing the levels of TREK1, CLDN1, CNDN3, and OCLN tight junction proteins and reducing the levels of IL-6, TNF-, and IFN- in the colon, resulting in reduced ASD-associated behaviors [365]

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