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Step 5: Mix gently After injecting all the water, do not shake the vial

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The liquid compound is not miscible with aliphatic and aromatic hydrocarbons and chlorinated hydrocarbons

Males have a greater density of BrdU/Sox2-ir cells in the dorsal DG at 2 h compared with females To understand whether there are differences between sexes in the time course of neural stem cell marker expression after mitosis, we examined the density of BrdU/Sox2-ir cells at 2 h, 24 h, 1w, 2w, and 3w after BrdU injection