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Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls

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Study participants were age -and sex-matched ( Figure 1B ) and all were infected with the Wuhan strain and recruited at ~12 months post the onset of acute infection ( Supplementary Table 1 )

You should also look into underlying GI conditions and drug interactions (such as steroids or laxatives) that could impair absorption