Imaging was performed using Plan Apo 60 oil (NA 1.4) objective and Andor DU-897 X-8536 camera of the inverted Nikon Ti microscope (Nikon Corporation, Chiyoda, Tokyo, Japan) setup
Ultimately, the oil must go through a deodorization process at high temperatures, typically around 204205 C (O'Brien 2012)
GHK-Cu's non-hormonal mechanism makes it suitable for women who can't or prefer not to use hormonal treatments
Kimborough CK, Griffiths CE, Finkel LJ, Hamilton TA, Bulengo SM, Ellis CN
doi:10.1002/mc.20741, PMID: 21319226 Yue P, Zhou Z, Khuri FR, Sun SY (2006) Depletion of intracellular glutathione contributes to JNK-mediated death receptor 5 upregulation and apoptosis induction by the novel synthetic triterpenoid methyl-2-cyano-3, 12-dioxooleana-1, 9-dien-28-oate (CDDO-Me)