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The plasmid plSAM-FVpE was confirmed by restriction enzyme digestion and gene sequencing ( Supplementary Figure 2C )

Frontiers in Endocrinology (Lausanne) , 9 , 476 Cheng, H., Ji, Z., Wang, Y., Li, S., Tang, T., Wang, F., Peng, C., Wu, X., Cheng, Y., Liu, Z., Ma, M., Wang, J., Huang, X., Wang, L., Qin, L., Liu, H., Chen, J., Zheng, R., Feng, C

Sonication involves exposing samples to ultrasonic waves which agitate the molecules and increase the solubility of peptide compounds in solution