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Standard preparation: Reduced glutathione standard (1 mmol/L) was gradient diluted using ultrapure water (e.g., 50 L of its standard was added to 950 L of ultrapure water, diluted to 50 mol/L and further gradient diluted to make a standard curve, i.e., 50/25/12.5/6.25/3.125 mol/L), and then used for data analysis by the standard curve method after subsequent testing with the samples to be tested