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Mice were treated with IHPC for a total of 14 days, followed by continuous treatment for another 14 days in 13% O 2 hypoxic conditions (Fig

Cytokine regulation When youre injured, or after surgery, your body releases proteins called cytokines

Regul Toxicol Pharmacol 120:104859

22 In addition, AARS1 senses intracellular lactate and serves as a bona fide lactyltransferase that directly uses lactate and ATP to catalyze the lactylation of Yes-associated protein (YAP) at K90 and the transcriptional enhanced associate domain (TEAD) at K108

After removing the magnetic beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)