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A double stainless steel guide cannula (26 gauge in diameter, Phymep, France) was implanted into the OB through stereotactic procedures (A/P: +4.8 mm from bregma, M/L: 1 mm and D/V: 1.7 mm from brain surface) after exposing and properly scrubbing the skull of the animal

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The structure of the HITI donor plasmid followed the previous report 5

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Daily movement targets of 8,000 to 10,000 steps distributed throughout the day rather than concentrated in single sessions