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Insert the needle into the AOD-9604 vial at a slight angle, with the needle tip pointed toward the inner wall of the vial

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In addition, the ubiquitin ligase RNF213 directly ubiquitylates lipopolysaccharides (LPS) upon vacuole rupture, followed by the recruitment of linear (M1) Ub assembly complex (LUBAC), which is responsible for the assembly of M1 ubiquitin chains