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Bohn et al., 1999, 2002), but we prefer to use the term tolerance for such prolonged exposure, qualified as acute (several hours, Cox et al., 1968) or long-term (days) to avoid confounding the mechanisms of rapid desensitization of MOR with potentially different mechanisms of tolerance

(2) 1000 mg metformin at t = 30 min and 0.9% saline at t = 60 and 0 min [Met (30 min)]