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RNA isolation and real-time RTqPCR Total RNA was isolated from cells or tissues using TRIzol Reagent and the Direct-zol RNA MiniPrep kit (Zymo Research) according to the manufacturers instructions

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THYROID INJURY AND ANTIGEN RELEASE It is accepted that certain types of injury to the thyroid are followed by the development of thyroid autoimmunity, including GD

pylori infection, changes in the intestinal microbiota, and intestinal immunity