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Cells were centrifuged, resuspended in HBSS with 15 mM HEPES, and sequentially passed through 100 m and 20 m mesh filters to isolate cell clusters between 20 and 100 m

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An example of this effect can be observed with bradykinin (purple box within Figure 8 ) (347, 348), and preload failure experienced in ME/CFS may be correlated with the endogenous vasodilator substances produced in skeletal muscles

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