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PBMCs from healthy donors were differentiated with GM-CSF (2050 ng/mL) for 6 days, followed by polarization with LPS (100 ng/mL) and IFN- (2050 ng/mL) for 12 days

A diagnostic trial utilizing physiologic doses of vitamin B12 (1 mcg daily) and observing daily reticulocyte counts after establishing a baseline may also be performed

Once the Semaglutide mixture is prepared, it should be refrigerated until ready for use

Some patients report noticing changes within 2-4 weeks, though individual responses vary