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RNA extraction, cDNA synthesis, and qPCR Total RNA was isolated from cardiac samples using TRIzol Reagent (Invitrogen, USA) at a ratio of 500 L per 50 mg of tissue, ensuring complete lysis and RNA release

GSH, GSSG, and the GSH:GSSG ratio were measured by enzymatic assay in bronchoalveolar lavage (BAL) fluid and in fresh epithelial cells from healthy controls (HCs), mild/moderate (M/M), and severe asthma (SA) patients

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FDAs scrutiny of these substances reflects a legitimate concern about ensuring that compounded products are safe and that clinical claims are substantiated but it also creates very real uncertainty for providers and patients who have relied on these compounds