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The apparent increase in sensitivity to IGF-1 was dependent on a functional IGF-1R since as IGF-1 induced Akt and ERK phosphorylation was markedly attenuated by the treatment with PPP (Figure 4f and 4g)

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Subsequently, the cells were washed with PBS and stained by C11-BODIPY 581/591 (5 M) for 20 min

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For reference, the FDA label dose of tesamorelin alone is 2 mg/day (legacy formulation) or 1.28 mg/day for the newer EGRIFTA WR formulation, and ipamorelin has been studied at roughly 100-300 mcg per dose in research settings