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PLoS One (2014) 9(8):e103798.10.1371/journal.pone.0103798 111 RizosECNtzaniEEPapanasNTsimihodimosVMitrogianniZMaltezosEet alCombination therapies of DPP4 inhibitors and GLP1 analogues with insulin in type 2 diabetic patients: a systematic review

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After three washes with PBS, the sections were pre-incubated in blocking solution (5% serum albumin, 2% normal goat serum, and 0.3% Triton X-100 in PBS) for 20 min at room temperature, then incubated with an anti-c-Fos rabbit antibody (#sc-52, 1:2000